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Compatibility Tips & np GTS Pipettes
This Compatibility Check helps you determine which pipette or filter tips from other manufacturers are compatible in terms of volume with the np GTS pipette from nerbe plus.
Simply select the manufacturer and the model/volume of your current pipette or filter tip to instantly display the compatible nerbe plus article numbers (including product description and color code). The results are grouped by volume and will lead you directly to the corresponding product page.
Furthermore, the corresponding np GreenTips are displayed, which—as a perfectly matched system component—guarantee maximum fit and optimal performance for your np GTS pipette.
(Icons zur Vergrößerung berühren. Artikel sind klickbar.)
Diese finden Sie direkt in den farblich markierten Kopfzeilen der Ergebnisse. Ein Klick auf die Pipetten-Abbildung führt Sie zu den detaillierten Eigenschaften – für eine optimale Passgenauigkeit und zuverlässige Laborergebnisse.
Die Passgenauigkeiten dieser Tabelle sind Empfehlungen der nerbe plus GmbH & Co. KG.
Die Übertragbarkeit ist daher von Ihnen selbst sehr sorgfältig zu prüfen. Ansprüche aus der Tabelle können nicht abgeleitet werden.
Precise pipetting: An introduction to dosing methodology
Pipetting is one of the most important and demanding methods in any laboratory, from medical diagnostics to biotechnological research. It involves the precise collection and dispensing of minute volumes of liquid (in the microliter range) using a pipette. The accuracy of this step is a fundamental prerequisite for the reproducibility and validity of all subsequent experiments and analyses.
| Feature | Single-channel pipette | Multichannel pipette |
|---|---|---|
| Number of Channels | One channel (one tip) | Multiple channels (usually 8 or 12 tips) |
| Field of Application | Standard Method for individual samples, serial measurements, sampling from bottles or irregularly arranged containers. | High throughput-applications that require filling many wells in microplates (96 or 384 format) require. |
| Working Method | Serial: Each sample is dosed individually. | Parallel: An entire row or column is dispensed at the same time. |
| Precision | Usually offers the highest mechanical precision per single dose. | Very good precision, but requires a uniform and dense installation of all tips for reliable results. |
| Efficiency | Lower efficiency with high sample throughput. | Significant time savings when working with panels. |
| Flexibility | High: Adjustable for all container types (tubes, bottles, plates). | Medium: Primarily designed for rasterization of microplates. |
The correct pipetting procedure (standard method)
The standard method is forward pipetting. Correct execution is essential for the precision of the dispensed volume.Phase 1: Preparation
- Volume setting: The desired volume must always be set slowly and precisely using the adjustment wheel.
- Tip assembly: The pipette tip must be placed firmly and tightly on the cone to prevent air leaks.
- Pre-wetting: The tip should be pre-wetted 2–3 times with the liquid to be pipetted. This moistens the inner walls of the tip and improves accuracy, especially with volatile solvents.
Phase 2: Aspiration
- First stop: The pipette plunger is pressed down to the first pressure point.
- Immersion: The tip is immersed vertically (or at a maximum angle of 20°) just a few millimeters into the liquid.
- Slow aspiration:The piston is released slowly and evenly to draw up the liquid. Releasing it too quickly creates negative pressure and leads to bubbles or inaccurate volume uptake.
Phase 3: Dispensing
- Target location: The tip is held against the inner wall of the target vessel.
- Dispensing: The plunger is pressed to the first stop to release the defined volume.
- Residual Emptying (Blow-out): The plunger is now pressed through to the second stop to remove all residual fluid remaining in the tip.
- Removal: The plunger must be held at the second stop while the tip is pulled out of the fluid along the vessel wall.
Phase 4: Completion
- Tip replacement: The tip must be disposed of immediately using the eject button to prevent cross-contamination of the next sample.
Qualitative factors for maximum accuracy
The following physical and technical aspects must be taken into account to ensure reproducible pipetting results:| Aspekt | Importance and Recommendation |
|---|---|
| Temperature Compensation | All components (pipette, tips, liquid) should ideally be at room temperature. |
| Vertical Alignment | When aspirating, the pipette should be held as vertically as possible. |
| Immersion Depth | The tip should be immersed minimally in the liquid (less than 2 mm). |
| Viscosity | Aspiration must be performed extremely slowly with viscous media. In this case, backward pipetting is often the method of choice. |
| Pipette Tip | Nur Use high-quality, compatible, and clean tips. Defective or wet tips will result in volume errors. |
Frequently Asked Questions on Dosing Methodology & Compatibility
Does tip compatibility affect accuracy during forward pipetting?
Absolutely. In forward pipetting (the standard method), an airtight seal between the pipette cone and the tip is essential. Even the slightest leak leads to incomplete pressure buildup, resulting in a lower aspirated volume than selected. Our compatibility check ensures that the mechanical fit eliminates these sources of error.
When should I use filter tips instead of standard pipette tips?
Filter tips should always be used when protection against aerosols and cross-contamination is required, such as in PCR, microbiology, or when handling radioactive samples. The integrated filter blocks the transfer of liquids and vapors into the pipette shaft, which also extends the lifespan of your pipette.
How does temperature influence the pipetting volume?
Temperature equilibration of the pipette, tip, and liquid is critical. Cold liquids are often aspirated in too high a volume, while warm liquids result in too low a volume. We recommend storing all components at room temperature and equilibrating the tip by pre-wetting it 2-3 times with the medium.
Why is low mounting force important for ergonomics?
High compatibility means the tip seals securely even with minimal pressure. This protects the pipette's seals and reduces the strain on the user's wrist (prevention of Repetitive Strain Injury or RSI). Our GreenTips are specifically optimized for easy mounting and ejection on the np GTS pipette.
How often should volume accuracy be checked for third-party combinations?
If you use nerbe plus tips on third-party pipettes, we recommend regular gravimetric testing (calibration) according to ISO 8655. Since pipette cones can wear over time, regular testing ensures that the specified compatibility continues to deliver full precision.
Are there special tips for viscous liquids?
For highly viscous media such as glycerol or oils, we recommend not only choosing the right tip but also using the reverse pipetting technique. In this method, a larger volume is aspirated than dispensed to compensate for capillary effects and surface tension residues inside the tip.
This Compatibility Check helps you determine which pipette or filter tips from other manufacturers are compatible in terms of volume with the np GTS pipette from nerbe plus.
Simply select the manufacturer and the model/volume of your current pipette or filter tip to instantly display the compatible nerbe plus article numbers (including product description and color code). The results are grouped by volume and will lead you directly to the corresponding product page.
Furthermore, the corresponding np GreenTips are displayed, which—as a perfectly matched system component—guarantee maximum fit and optimal performance for your np GTS pipette.
(Icons zur Vergrößerung berühren. Artikel sind klickbar.)
Diese finden Sie direkt in den farblich markierten Kopfzeilen der Ergebnisse. Ein Klick auf die Pipetten-Abbildung führt Sie zu den detaillierten Eigenschaften – für eine optimale Passgenauigkeit und zuverlässige Laborergebnisse.
Die Passgenauigkeiten dieser Tabelle sind Empfehlungen der nerbe plus GmbH & Co. KG.
Die Übertragbarkeit ist daher von Ihnen selbst sehr sorgfältig zu prüfen. Ansprüche aus der Tabelle können nicht abgeleitet werden.
Precise pipetting: An introduction to dosing methodology
Pipetting is one of the most important and demanding methods in any laboratory, from medical diagnostics to biotechnological research. It involves the precise collection and dispensing of minute volumes of liquid (in the microliter range) using a pipette. The accuracy of this step is a fundamental prerequisite for the reproducibility and validity of all subsequent experiments and analyses.
| Feature | Single-channel pipette | Multichannel pipette |
|---|---|---|
| Number of Channels | One channel (one tip) | Multiple channels (usually 8 or 12 tips) |
| Field of Application | Standard Method for individual samples, serial measurements, sampling from bottles or irregularly arranged containers. | High throughput-applications that require filling many wells in microplates (96 or 384 format) require. |
| Working Method | Serial: Each sample is dosed individually. | Parallel: An entire row or column is dispensed at the same time. |
| Precision | Usually offers the highest mechanical precision per single dose. | Very good precision, but requires a uniform and dense installation of all tips for reliable results. |
| Efficiency | Lower efficiency with high sample throughput. | Significant time savings when working with panels. |
| Flexibility | High: Adjustable for all container types (tubes, bottles, plates). | Medium: Primarily designed for rasterization of microplates. |
The correct pipetting procedure (standard method)
The standard method is forward pipetting. Correct execution is essential for the precision of the dispensed volume.Phase 1: Preparation
- Volume setting: The desired volume must always be set slowly and precisely using the adjustment wheel.
- Tip assembly: The pipette tip must be placed firmly and tightly on the cone to prevent air leaks.
- Pre-wetting: The tip should be pre-wetted 2–3 times with the liquid to be pipetted. This moistens the inner walls of the tip and improves accuracy, especially with volatile solvents.
Phase 2: Aspiration
- First stop: The pipette plunger is pressed down to the first pressure point.
- Immersion: The tip is immersed vertically (or at a maximum angle of 20°) just a few millimeters into the liquid.
- Slow aspiration:The piston is released slowly and evenly to draw up the liquid. Releasing it too quickly creates negative pressure and leads to bubbles or inaccurate volume uptake.
Phase 3: Dispensing
- Target location: The tip is held against the inner wall of the target vessel.
- Dispensing: The plunger is pressed to the first stop to release the defined volume.
- Residual Emptying (Blow-out): The plunger is now pressed through to the second stop to remove all residual fluid remaining in the tip.
- Removal: The plunger must be held at the second stop while the tip is pulled out of the fluid along the vessel wall.
Phase 4: Completion
- Tip replacement: The tip must be disposed of immediately using the eject button to prevent cross-contamination of the next sample.
Qualitative factors for maximum accuracy
The following physical and technical aspects must be taken into account to ensure reproducible pipetting results:| Aspekt | Importance and Recommendation |
|---|---|
| Temperature Compensation | All components (pipette, tips, liquid) should ideally be at room temperature. |
| Vertical Alignment | When aspirating, the pipette should be held as vertically as possible. |
| Immersion Depth | The tip should be immersed minimally in the liquid (less than 2 mm). |
| Viscosity | Aspiration must be performed extremely slowly with viscous media. In this case, backward pipetting is often the method of choice. |
| Pipette Tip | Nur Use high-quality, compatible, and clean tips. Defective or wet tips will result in volume errors. |
Frequently Asked Questions on Dosing Methodology & Compatibility
Does tip compatibility affect accuracy during forward pipetting?
Absolutely. In forward pipetting (the standard method), an airtight seal between the pipette cone and the tip is essential. Even the slightest leak leads to incomplete pressure buildup, resulting in a lower aspirated volume than selected. Our compatibility check ensures that the mechanical fit eliminates these sources of error.
When should I use filter tips instead of standard pipette tips?
Filter tips should always be used when protection against aerosols and cross-contamination is required, such as in PCR, microbiology, or when handling radioactive samples. The integrated filter blocks the transfer of liquids and vapors into the pipette shaft, which also extends the lifespan of your pipette.
How does temperature influence the pipetting volume?
Temperature equilibration of the pipette, tip, and liquid is critical. Cold liquids are often aspirated in too high a volume, while warm liquids result in too low a volume. We recommend storing all components at room temperature and equilibrating the tip by pre-wetting it 2-3 times with the medium.
Why is low mounting force important for ergonomics?
High compatibility means the tip seals securely even with minimal pressure. This protects the pipette's seals and reduces the strain on the user's wrist (prevention of Repetitive Strain Injury or RSI). Our GreenTips are specifically optimized for easy mounting and ejection on the np GTS pipette.
How often should volume accuracy be checked for third-party combinations?
If you use nerbe plus tips on third-party pipettes, we recommend regular gravimetric testing (calibration) according to ISO 8655. Since pipette cones can wear over time, regular testing ensures that the specified compatibility continues to deliver full precision.
Are there special tips for viscous liquids?
For highly viscous media such as glycerol or oils, we recommend not only choosing the right tip but also using the reverse pipetting technique. In this method, a larger volume is aspirated than dispensed to compensate for capillary effects and surface tension residues inside the tip.